Yousuke Takaoka
Development of random loop-stapling peptide for ubiquitin code analyses
 |
Yousuke Takaoka, PhDDepartment of Chemistry, Graduate School of Science, Tohoku University |
|---|
Research summary
The plant hormone jasmonates (JA) mediates plant defense responses against environmental stresses such as herbivore and pathogen attacks. 7-iso-(+)-jasmonoyl-L-isoleucine (JA-Ile), an active form of JA, induces protein-protein interaction (PPI) between COI1, the F-box protein in the SCFCOI1 ubiquitin E3 ligase complex, and the JAZ transcriptional repressor protein. This interaction causes JAZ degradation via proteasome, leading to plant defense responses, as well as growth inhibition or senescence. COI1 and 13 subtypes of JAZ are encoded in the genome of Arabidopsis thaliana, and JA-Ile can induce PPI between COI1 and most of JAZ subtypes to cause various physiological responses at the same time. However, the detailed function or ubiquitination sites/patterns of most of JAZ subtypes have not been elucidated so far, partially due to the genetic redundancy of JAZ genes. We developed comprehensive detection methods for PPI between COI1 and JAZ or JAZ and transcriptional factors (TFs), according to the previously reported crystal structures. By using these methods, we succeeded in the rational design of JAZ-subtype selective agonist that induces PPI between specific JAZ and COI1. Furthermore, JAZ-based functional ligands for COI1 or JA-related TFs will also be developed, those would be useful and novel chemical tools for the complexed JA signaling or ubiquitination analyses of JAZ in planta.
Publications
- Takaoka Y, Miyagawa S, Nakamura A, Egoshi S, Tsukiji S, *Ueda M.
Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana.
Sci. Rep. 10, 5333 (2020)
PMID: 32210301 - Suzuki K, *Takaoka Y, Ueda M.
Rational design of a stapled JAZ9 peptide inhibiting protein-protein interaction of a plant transcription factor.
RSC. Chem. Biol. AOP (2021)
(This paper was selected as an inside cover picture in RSC Chemical biology)
Former Publications
- Takaoka Y, Ojida A, *Hamachi I.
Protein Organic Chemistry and Application for Labeling and Engineering in Live-Cell Systems.
Angew. Chem. Int. Ed. 52, 4088-4106 (2013)
PMID: 23426903 - Yoshii T, Mizusawa K, *Takaoka Y, *Hamachi I.
Intracellular Protein-responsive Supramolecules: Protein Sensing and In-cell Construction of Inhibitor Assay System.
J. Am. Chem. Soc. 136, 16635-16642 (2014)
PMID: 25361466 - Takaoka Y, Uchinomiya S, Kobayashi D, Endo M, Hayashi T, Fukuyama Y, Hayasaka H, Miyasaka M, Ueda T, Shimada I, *Hamachi I.
Endogenous Membrane Receptor Labeling by Reactive Cytokines and Growth Factors to Chase Their Dynamics in Live Cells.
Chem 4, 1451-1464 (2018) - *Takaoka Y, Nagumo K, Azizah IN, Oura S, Iwahashi M, Kato N, *Ueda M.
A Comprehensive in vitro fluorescence anisotropy assay system for screening ligands of the jasmonate COI1-JAZ co-receptor in plants.
J. Biol. Chem. 294, 5074-5081 (2019)
PMID: 30728246 - Takaoka Y, Iwahashi M, Chini A, Saito H, Ishimaru Y, Egoshi S, Kato N, Tanaka M, Bashir K, Seki M, Solano R, *Ueda M.
A rationally designed JAZ subtype-selective agonist of jasmonate perception.
Nat. Commun. 9, 3654 (2018)
PMID: 30194307 - Takaoka Y, Iwahashi M, Chini A, Saito H, Ishimaru Y, Egoshi S, Kato N, Tanaka M, Bashir K, Seki M, Solano R, *Ueda M.
A rationally designed JAZ subtype-selective agonist of jasmonate perception.
Nat. Commun. 9, 3654 (2018)
PMID: 30194307 - *Takaoka Y, Nagumo K, Azizah IN, Oura S, Iwahashi M, Kato N, *Ueda M.
A comprehensive in vitro fluorescence anisotropy assay system for screening ligands of the jasmonate COI1–JAZ co-receptor in plants.
J. Biol. Chem. 294, 5074-5081 (2019)
PMID: 30728246